Tan, Ying; You, Changjun; Park, Jiyeong; Kim, Hyun Suk; Guo, Su; Scharer, Orlando D.; Wang, Yinsheng published the artcile< Transcriptional Perturbations of 2,6-Diaminopurine and 2-Aminopurine>, Recommanded Product: 7H-Purin-2-amine, the main research area is transcription diaminopurine aminopurine.
2,6-Diaminopurine (Z) is a naturally occurring adenine (A) analog that bacteriophages employ in place of A in their genetic alphabet. Recent discoveries of biogenesis pathways of Z in bacteriophages have stimulated substantial research interest in this DNA modification. Here, the authors systematically examined the effects of Z on the efficiency and fidelity of DNA transcription. The authors’ results showed that Z exhibited no mutagenic yet substantial inhibitory effects on transcription mediated by purified T7 RNA polymerase and by human RNA polymerase II in HeLa nuclear extracts and in human cells. A structurally related adenine analog, 2-aminopurine (2AP), strongly blocked T7 RNA polymerase but did not impede human RNA polymerase ii in vitro or in human cells, where no mutant transcript could be detected. The lack of mutagenic consequence and the presence of a strong blockage effect of Z on transcription suggest a role of Z in transcriptional regulation. Z is also subjected to removal by transcription-coupled nucleotide-excision repair (TC-NER), but not global-genome NER in human cells. The authors’ findings provide new insight into the effects of Z on transcription and its potential biol. functions.
ACS Chemical Biology published new progress about Homo sapiens. 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Recommanded Product: 7H-Purin-2-amine.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem