Ren, Jing; Ding, Liang; Zhang, Dongya; Shi, Guoping; Xu, Qianyun; Shen, Sunan; Wang, Yaping; Wang, Tingting; Hou, Yayi published the artcile< Carcinoma-associated fibroblasts promote the stemness and chemoresistance of colorectal cancer by transferring exosomal lncRNA H19>, Electric Literature of 6823-69-4, the main research area is colorectal cancer fibroblast stemness chemoresistance lncRNA H19; CAFs; CRC; H19; chemoresistance; stemness.
In this study, we further investigated the underlying tumor-promoting roles of CAFs and the mol. mediators involved in these processes. Methods: The AOM/DSS-induced colitis-associated cancer (CAC) mouse model was established, and RNA sequencing was performed. SW480 cells with H19 stably knocked down were used to establish a xenograft model. The indicated protein levels in xenograft tumor tissues were confirmed by immunohistochem. assay, and cell apoptosis was analyzed by TUNEL apoptosis assay. The AldeRed ALDH detection assay was performed to detect intracellular aldehyde dehydrogenase (ALDH) enzyme activity. Results: H19 was highly expressed in the tumor tissues of CAC mice compared with the expression in normal colon tissues. Moreover, H19 was associated with the stemness of colorectal cancer stem cells (CSCs) in CRC specimens. Of note, H19 was enriched in CAF-derived conditioned medium and exosomes, which in turn promoted the stemness of CSCs and the chemoresistance of CRC cells in vitro and in vivo. Conclusion: CAFs promote the stemness and chemoresistance of CRC by transferring exosomal H19. H19 activated the β-catenin pathway via acting as a competing endogenous RNA sponge for miR-141, while miR-141 inhibited the stemness of CRC cells. Our findings indicate that H19 expressed by CAFs of the colorectal tumor stroma contributes to tumor development and chemoresistance.
Theranostics published new progress about Animal gene, HSP70 Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 6823-69-4 belongs to class imidazoles-derivatives, and the molecular formula is C30H30Cl2N6O2, Electric Literature of 6823-69-4.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem