Paterson, Kyle A.; Arlt, Jochen; Jones, Anita C. published the artcile< Dynamic and static quenching of 2-aminopurine fluorescence by the natural DNA nucleotides in solution>, Related Products of 452-06-2, the main research area is nucleoside monophosphate base stacking fluorescence quenching charge transfer.
2-Aminopurine (2AP)is a responsive fluorescent base analog that is used widely as a probe of the local mol. environment in DNA. The ability of 2AP to report changes in local conformation and base-stacking interactions arises from the efficient quenching of its fluorescence by the natural DNA bases. However, the mechanism of this inter-base quenching remains imperfectly understood. Two previous studies of the collisional quenching of 2AP by the natural bases, in different buffer solutions, showed that dynamic quenching efficiency depends on the identity of the natural base, but disagreed on the relative quenching efficiencies of the bases. We report a comprehensive investigation of interbase quenching of 2AP by the natural nucleoside monophosphates(NMPs), replicating the buffer conditions used in the previous studies. Using time-resolved fluorescence measurements to distinguish between dynamic and static quenching, we find that the dynamic quenching rate constants of the different bases show a consistent trend across both buffers, and this is in line with a charge-transfer mechanism. Time-resolved measurements also provide insight into static quenching, revealing formation of 2AP-NMP ground-state complexes in which 2AP displays a very short fluorescence lifetime, comparable to that seen in oligonucleotides. In these complexes, the dependence of the rate of quenching on the partner base also supports a charge-transfer mechanism.
Methods and Applications in Fluorescence published new progress about Buffers. 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Related Products of 452-06-2.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem