Nejad, Maryam Imani; Price, Nathan E.; Haldar, Tuhin; Lewis, Calvin; Wang, Yinsheng; Gates, Kent S. published the artcile< Interstrand DNA cross-links derived from reaction of a 2-aminopurine residue with an abasic site>, Electric Literature of 452-06-2, the main research area is interstrand DNA crosslink derived reaction aminopurine abasic.
Efficient methods for the site-specific installation of structurally-defined interstrand crosslinks in duplex DNA may be useful in a wide variety of fields. The work described here developed a high-yield synthesis of chem. stable interstrand crosslinks resulting from a reductive amination reaction between an abasic site and the noncanonical nucleobase 2-aminopurine in duplex DNA. Results from footprinting, LC-MS, and stability studies support the formation of an N2-alkylamine attachment between the 2-aminopurine residue and the Ap site. The reaction performs best when the 2-aminopurine residue on the opposing strand is offset 1 nt to the 5′-side of the abasic site. The crosslink confers substantial resistance to thermal denaturation (melting). The crosslinking reaction is fast (complete in 4 h), employs only com. available reagents, and can be used to generate crosslinked duplexes in sufficient quantities for biophys., structural, and DNA repair studies.
ACS Chemical Biology published new progress about DNA damage. 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Electric Literature of 452-06-2.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem