Ma, Fei; Wang, Ting-ting; Jiang, Longhe; Zhang, Chun-yang published the artcile< Ultrasensitive detection of telomerase activity in lung cancer cells with quencher-free molecular beacon-assisted quadratic signal amplification>, Recommanded Product: 7H-Purin-2-amine, the main research area is telomerase detection mol beacon quadratic signal amplification; Fluorescence detection; Lung cancer; Molecular beacon; Signal amplification; Telomerase.
Telomerase is an important biomarker for cancer diagnosis and a valuable target for cancer therapy. Most of the reported telomerase assays suffer from the repeating thermal cycles, laborious protocols, expensive instruments and the limited sensitivity. To solve these issues, we develop a new telomerase assay based on telomerization-driven release of fluorescent 2-aminopurine. We designed a 2-aminopurine mol. beacon in which the fluorescence of 2-aminopurine is quenched due to stacking interaction effect without the use of extra quenchers. The presence of target telomerase can open the 2-aminopurine mol. beacon, triggering a quadratic signal amplification reaction to digest abundant 2-aminopurine mol. beacons, releasing large amounts of free 2-aminopurine mols. with strong fluorescence emission. Due to the inherent low background signal of 2-aminopurine mol. beacon and the highly efficient telomerization-driven quadratic signal amplification, this method exhibits high sensitivity with a limit of detection equivalent to 1 human lung cancer A549 cell and a large dynamic range of 4 orders of magnitude from 1 to 10000 cells. Moreover, this method can be further applied for telomerase inhibition assay and the discrimination of cancer cells from normal cells, holding great potential in telomerase-related clin. diagnosis and drug discovery.
Analytica Chimica Acta published new progress about Cancer diagnosis. 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Recommanded Product: 7H-Purin-2-amine.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem