Li, Quan; Li, Hanhao; Liang, Jinlian; Mei, Jiaxin; Cao, Zhen; Zhang, Lei; Luo, Jiao; Tang, Yan; Huang, Rufei; Xia, Huan; Zhang, Qihao; Xiang, Qi; Yang, Yan; Huang, Yadong published the artcile< Sertoli cell-derived exosomal MicroRNA-486-5p regulates differentiation of spermatogonial stem cell through PTEN in mice>, Reference of 6823-69-4, the main research area is sertoli cell exosomal miR4865p differentiation PTEN spermatogonial stem; Sertoli cells; differentiation; exosome; miR-486-5p; spermatogonial stem cells.
Self-renewal and differentiation of spermatogonial stem cell (SSC) are critical for male fertility and reproduction, both of which are highly regulated by testicular microenvironment. Exosomal miRNAs have emerged as new components in intercellular communication. However, their roles in the differentiation of SSC remain unclear. Here, we observed miR-486-5p enriched in Sertoli cell and Sertoli cell-derived exosomes. The exosomes mediate the transfer of miR-486-5p from Sertoli cells to SSCs. Exosomes release miR-486-5p, thus up-regulate expression of Stra8 (stimulated by retinoic acid 8) and promote differentiation of SSC. And PTEN was identified as a target of miR-486-5p. Overexpression of miR-486-5p in SSCs down-regulates PTEN expression, which up-regulates the expression of STRA8 and SYCP3, promotes SSCs differentiation. In addition, blocking the exosome-mediated transfer of miR-486-5p inhibits differentiation of SSC. Our findings demonstrate that miR-486-5p acts as a communication mol. between Sertoli cells and SSCs in modulating differentiation of SSCs. This provides a new insight on mol. mechanisms that regulates SSC differentiation and a basis for the diagnosis, treatment, and prevention of male infertility.
Journal of Cellular and Molecular Medicine published new progress about CD81 antigens Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 6823-69-4 belongs to class imidazoles-derivatives, and the molecular formula is C30H30Cl2N6O2, Reference of 6823-69-4.
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Imidazole | C3H4N2 – PubChem