Maji, Abhishek;Pal, Siddhartha;Lohar, Somenath;Mukhopadhyay, Subhra Kanti;Chattopadhyay, Pabitra published 《A new turn-on benzimidazole-based greenish-yellow fluorescent sensor for Zn2+ ions at biological pH applicable in cell imaging》. The research results were published in《New Journal of Chemistry》 in 2017.Formula: C13H11N3 The article conveys some information:
A newly designed and structurally characterized benzimidazole containing compound, 2,4-di-tert-butyl-6-(5,6-dihydro benzo[4,5] imidazo [1,2-c] quinazolin-6-yl)-phenol (HL), behaves as a turn-on fluorescent sensor selective for Zn2+ ions at as low as 39.91 nM within a very short responsive time (15-20 s) in 5 mM HEPES buffer (DMSO/water: 1/5, volume/volume) at biol. pH. Thorough exptl. and theor. (DFT) studies indicate the occurrence of greenish yellow fluorescence through a chelation enhanced fluorescence (CHEF) process. Using this organic moiety (HL) as a probe, almost no interference of other competitive ions in the detection of Zn2+ ions was observed The reaction of HL with Zn2+ led to the in situ formation of a tridentate monobasic ligand (HL1) to produce the complex as [Zn(L1)2] through a [1,5] sigmatropic-type shift of HL prior to metal coordination. HL is also capable of detecting the intercellular distribution of Zn2+ ions in Candida sp. cells under a fluorescence microscope by developing the image. And 2-(1H-Benzo[d]imidazol-2-yl)aniline (cas: 5805-39-0) was used in the research process.
2-(1H-Benzo[d]imidazol-2-yl)aniline(cas:5805-39-0 Formula: C13H11N3) is a chemical reagent used in the synthesis of small molecule inhibitors targeting ubiquitin-like domains for treatments of diseases caused by the cellular accumulation of damaged proteins.
Reference:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem